Aim To perform a genetic characterization of 7 skeletonsfrom medieval age found in a burial site in the AragonesePyrenees.Methods Allele frequencies of autosomal short tandemrepeats (STR) loci were determined by 3 different STR systems.Mitochondrial DNA (mtDNA) and Y-chromosomehaplogroups were determined by sequencing of the hypervariablesegment 1 of mtDNA and typing of phylogeneticY chromosome single nucleotide polymorphisms (YSNP)markers, respectively. Possible familial relationshipswere also investigated.Results Complete or partial STR profiles were obtained in3 of the 7 samples. Mitochondrial DNA haplogroup wasdetermined in 6 samples, with 5 of them correspondingto the haplogroup H and 1 to the haplogroup U5a. Ychromosomehaplogroup was determined in 2 samples,corresponding to the haplogroup R. In one of them, thesub-branch R1b1b2 was determined. mtDNA sequencesindicated that some of the individuals could be maternallyrelated, while STR profiles indicated no direct family relationships.Conclusions Despite the antiquity of the samples andgreat difficulty that genetic analyses entail, the combineduse of autosomal STR markers, Y-chromosome informativeSNPs, and mtDNA sequences allowed us to genotype agroup of skeletons from the medieval age.
展开▼
